The importance of ceramide headgroup for lipid localisation in skin lipid models

https://doi.org/10.1016/j.bbamem.2022.183886Get rights and content
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Highlights

Lipid subclasses selectively localize within the trilayer unit cell of the long periodicity phase.

Sphingosine chain of CER NS is in the outer layers of the trilayer unit cell.

When CER NS is partly replaced by sphingosine, the tails remain in the outer layers of the unit cell.

When CER NS is partly replaced by fatty acids, the fatty acid is evenly distributed in the unit cell.

Sphingosine head group is crucial in directing the sphingosine chain to the unit cell outer layers.

Abstract

The stratum corneum's lipid matrix is a critical for the skin's barrier function and is primarily composed of ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs). The lipids form a long periodicity phase (LPP), a unique trilayer unit cell structure. An enzyme driven pathway is implemented to synthesize these key lipids. If these enzymes are down- or upregulated as in inflammatory diseases, the final lipid composition is affected often altering the barrier function. In this study, we mimicked down regulation of enzymes involved in the synthesis of the sphingosine and CER amide bond. In a LPP lipid model, we substituted CER N-(tetracosanoyl)-sphingosine (CER NS) with either i) FFA C24 and free sphingosine, to simulate the loss of the CER amide bond, or ii) with FFA C24 and C18 to simulate the loss of the sphingosine headgroup. Our study shows the lipids in the LPP would not phase separate until at least 25% of the CER NS is substituted keeping the lateral packing and conformational ordering unaltered. Neutron diffraction studies showed that free sphingosine chains localized at the outer layers of the unit cell, while the remaining CER NS head group was concentrated in the inner headgroup layers. However, when FFA C18 was inserted, CER NS was dispersed throughout the LPP, resulting in an even distribution between the inner and outer water layers. The presented results highlight the importance of the CER NS headgroup structure and its interaction in combination with the carbon chain invariability for optimal lipid arrangement.