Cover for Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls

Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls

Book2014

Authors:

John T. Corthell

Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls

Book2014

 

Cover for Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls

Authors:

John T. Corthell

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Book description

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scienti ... read full description

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  2. Book chapterAbstract only

    Chapter 1 - General Notes

    Pages 1-9

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    Chapter 2 - DNA and RNA Extraction Protocols

    Pages 11-19

  4. Book chapterAbstract only

    Chapter 3 - Agarose Gel Electrophoresis

    Pages 21-25

  5. Book chapterAbstract only

    Chapter 4 - Reverse Transcription (RT) and Polymerase Chain Reaction (PCR)

    Pages 27-40

  6. Book chapterAbstract only

    Chapter 5 - Antibodies and Titrations

    Pages 41-46

  7. Book chapterAbstract only

    Chapter 6 - Protein Extraction

    Pages 47-53

  8. Book chapterAbstract only

    Chapter 7 - Immunoblotting (Western Blot)

    Pages 55-75

  9. Book chapterAbstract only

    Chapter 8 - Immunoprecipitation

    Pages 77-81

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    Chapter 9 - Perfusion and Immersion Fixation

    Pages 83-90

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    Chapter 10 - Immunohistochemistry

    Pages 91-103

  12. Book chapterAbstract only

    Chapter 11 - In Situ Hybridization

    Pages 105-111

  13. Book chapterNo access

    References

    Pages 113-114

About the book

Description

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials.

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials.

Key Features

  • Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding
  • Offers many details not present in other protocol books
  • Describes relevant controls for each technique and what those controls mean
  • Chapters include references (key articles, books, protocols) for additional study
  • Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules
  • Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding
  • Offers many details not present in other protocol books
  • Describes relevant controls for each technique and what those controls mean
  • Chapters include references (key articles, books, protocols) for additional study
  • Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules

Details

ISBN

978-0-12-801461-5

Language

English

Published

2014

Copyright

Copyright © 2014 Elsevier Inc. All rights reserved.

Imprint

Academic Press

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Authors

John T. Corthell