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Chapter 1 - Strategies to improve the expression and solubility of recombinant proteins in E. coli
Niharika Nag, Heena Khan and Timir Tripathi
Pages 1-12 - Book chapterAbstract only
Chapter 2 - Advances in heterologous protein expression strategies in yeast and insect systems
Meenakshi Singh, Smita Gupta, ... Sudhir Kumar Singh
Pages 13-30 - Book chapterAbstract only
Chapter 3 - Methods for transient expression and purification of monoclonal antibodies in mammalian cells
Suchitra Kamle, Dawei Li, ... Jack A. Elias
Pages 31-39 - Book chapterAbstract only
Chapter 4 - Methods for recombinant production and purification of intrinsically disordered proteins
Steffen P. Graether
Pages 41-48 - Book chapterAbstract only
Chapter 5 - Methods to determine the oligomeric structure of proteins
Purna Bahadur Chetri, Heena Khan and Timir Tripathi
Pages 49-76 - Book chapterAbstract only
Chapter 6 - Multimodal methods to study protein aggregation and fibrillation
Maria Georgina Herrera, Marco Giampà, ... Veronica Isabel Dodero
Pages 77-102 - Book chapterAbstract only
Chapter 7 - Experimental methods to study the thermodynamics of protein–protein interactions
Santanu Sasidharan, Niharika Nag, ... Prakash Saudagar
Pages 103-114 - Book chapterAbstract only
Chapter 8 - Experimental methods to study the kinetics of protein–protein interactions
Abhay Narayan Singh, Kristijan Ramadan and Shalini Singh
Pages 115-124 - Book chapterAbstract only
Chapter 9 - Computational techniques for studying protein-protein interactions
Khattab Al-Khafaji and Tugba Taskin-Tok
Pages 125-135 - Book chapterAbstract only
Chapter 10 - Experimental methods to study protein–nucleic acid interactions
Roberto Giambruno, Jakob Rupert and Elsa Zacco
Pages 137-161 - Book chapterAbstract only
Chapter 11 - Advanced computational tools for quantitative analysis of protein–nucleic acid interfaces
Sunandan Mukherjee and Chandran Nithin
Pages 163-180 - Book chapterAbstract only
Chapter 12 - Experimental techniques to study protein dynamics and conformations
Akshita Gupta, Anamika Singh, ... Pradeep Sharma
Pages 181-197 - Book chapterAbstract only
Chapter 13 - Computational techniques to study protein dynamics and conformations
Anil Mhashal, Agusti Emperador and Laura Orellana
Pages 199-212 - Book chapterAbstract only
Chapter 14 - Application of circular dichroism spectroscopy in studying protein folding, stability, and interaction
Md Anzarul Haque, Punit Kaur, ... Md Imtaiyaz Hassan
Pages 213-224 - Book chapterAbstract only
Chapter 15 - Studying protein-folding dynamics using single-molecule fluorescence methods
Narattam Mandal, Krishnananda Chattopadhyay and Achinta Sannigrahi
Pages 225-236 - Book chapterAbstract only
Chapter 16 - Advances in liquid-state NMR spectroscopy to study the structure, function, and dynamics of biomacromolecules
Priyanka Aggarwal, Pooja Kumari and Neel Sarovar Bhavesh
Pages 237-266 - Book chapterAbstract only
Chapter 17 - In-cell NMR spectroscopy: A tool to study cellular structure biology
Vijay Kumar
Pages 267-276 - Book chapterAbstract only
Chapter 18 - Current trends in membrane protein crystallography
Koomity V. Nageswar, Mansi Sharma, ... Appu K. Singh
Pages 277-290 - Book chapterAbstract only
Chapter 19 - Advances in sample preparation and data processing for single-particle cryo-electron microscopy
Anshul Assaiya, Suparna Bhar and Janesh Kumar
Pages 291-309 - Book chapterAbstract only
Chapter 20 - Advanced mass spectrometry-based methods for protein molecular-structural biologists
Joanna Bons, Jacob Rose, ... Birgit Schilling
Pages 311-326 - Book chapterAbstract only
Chapter 21 - Developments, advancements, and contributions of mass spectrometry in omics technologies
Saravanan Kumar
Pages 327-356 - Book chapterAbstract only
Chapter 22 - Role of structural biology methods in drug discovery
Fouzia Nasim and Insaf Ahmed Qureshi
Pages 357-371 - Book chapterAbstract only
Chapter 23 - Prediction, validation, and analysis of protein structures: A beginner’s guide
Santanu Sasidharan and Prakash Saudagar
Pages 373-385 - Book chapterAbstract only
Chapter 24 - Advances in structure-based virtual screening for drug discovery
Olujide O. Olubiyi, Suman Samantray and Alexander-Maurice Illig
Pages 387-404 - Book chapterAbstract only
Chapter 25 - Methods and applications of machine learning in structure-based drug discovery
Madhumathi Sanjeevi, Prajna N. Hebbar, ... Kanagaraj Sekar
Pages 405-437 - Book chapterAbstract only
Chapter 26 - Molecular dynamics simulations: Principles, methods, and applications in protein conformational dynamics
Aditya K. Padhi, Matej Janežič and Kam Y.J. Zhang
Pages 439-454 - Book chapterAbstract only
Chapter 27 - Applications of molecular dynamics simulations in drug discovery
Xubo Lin
Pages 455-465 - Book chapterAbstract only
Chapter 28 - Envisaging the conformational space of proteins by coupling machine learning and molecular dynamics
Murali Aarthy and Sanjeev Kumar Singh
Pages 467-475 - Book chapterAbstract only
Chapter 29 - Immunoinformatics and reverse vaccinology methods to design peptide-based vaccines
Vinita Sharma, Satyendra Singh, ... Vijay Kumar Prajapati
Pages 477-487 - Book chapterAbstract only
Chapter 30 - Computational methods to study intrinsically disordered proteins
Prateek Kumar, Aparna Bhardwaj, ... Rajanish Giri
Pages 489-504 - Book chapterAbstract only
Chapter 31 - Experimental methods to study intrinsically disordered proteins
Niharika Nag, Purna Bahadur Chetri, ... Timir Tripathi
Pages 505-533 - Book chapterAbstract only
Chapter 32 - Analysis of structure and dynamics of intrinsically disordered regions in proteins using solution NMR methods
Nikita V. Saibo, Snigdha Maiti, ... Soumya De
Pages 535-550 - Book chapterAbstract only
Chapter 33 - Methods to study the effect of solution variables on the conformational dynamics of intrinsically disordered proteins
Hakan Alici, Orkun Hasekioglu, ... Orkid Coskuner-Weber
Pages 551-563 - Book chapterAbstract only
Chapter 34 - Molecular simulations to study IDP-IDP interactions and their complexes
Kota Kasahara
Pages 565-574 - Book chapterAbstract only
Chapter 35 - Exploring large-scale protein function using systematic mutant analysis
Amrita Arpita Padhy, Subhashree Sahoo, ... Parul Mishra
Pages 575-588 - Book chapterAbstract only
Chapter 36 - Approaches and methods to study cell signaling: Linguistics of cellular communication
Siddharth Neog and Vishal Trivedi
Pages 589-623 - Book chapterAbstract only
Chapter 37 - Methods to study systems biology of signaling networks: A case study of NSCLC
Nikhil H. Samarth and Shailza Singh
Pages 625-634 - Book chapterAbstract only
Chapter 38 - Advancements in the analysis of protein post-translational modifications
Sandip Mukherjee, Ritesh Kumar, ... K. Venuprasad
Pages 635-640 - Book chapterAbstract only
Chapter 39 - Protein engineering: Methods and applications
Saurabh Bansal and Bishwajit Kundu
Pages 641-668 - Book chapterAbstract only
Chapter 40 - Designer 3D-DNA nanodevices: Structures, functions, and cellular applications
Anjali Rajwar, Payal Vaswani, ... Dhiraj Bhatia
Pages 669-676 - Book chapterNo access
Index
Pages 677-685
About the book
Description
Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering.
It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application.
Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering.
It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application.
Key Features
- Presents a thorough overview of the latest and emerging methods and technologies for protein study
- Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy
- Includes computational and machine learning methods
- Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins
- Presents a thorough overview of the latest and emerging methods and technologies for protein study
- Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy
- Includes computational and machine learning methods
- Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins
Details
ISBN
978-0-323-90264-9
Language
English
Published
2022
Copyright
Copyright © 2022 Elsevier Inc. All rights reserved.
Imprint
Academic Press