Browse content
Table of contents
Actions for selected chapters
- Full text access
- Book chapterAbstract only
Chapter 1 - Introduction to molecular biology techniques
Pages 1-6 - Book chapterAbstract only
Chapter 2 - Agarose gel electrophoresis
Pages 7-12 - Book chapterAbstract only
Chapter 3 - SDS-polyacrylamide gel electrophoresis
Pages 13-20 - Book chapterAbstract only
Chapter 4 - Immunoelectrophoresis
Pages 21-25 - Book chapterAbstract only
Chapter 5 - Plasmid DNA isolation using alkaline lysis method from E. coli DH5α cells
Pages 27-32 - Book chapterAbstract only
Chapter 6 - Restriction digestion of plasmid DNA
Pages 33-36 - Book chapterAbstract only
Chapter 7 - Genomic DNA extraction from the plant leaves using the CTAB method
Pages 37-44 - Book chapterAbstract only
Chapter 8 - Genomic DNA extraction from silkworm using modified phenol:chloroform method (Suzuki et al., 1972)
Pages 45-50 - Book chapterAbstract only
Chapter 9 - Genomic DNA extraction from Fungal Mycelium using the modified Cenis, 1992 method
Pages 51-56 - Book chapterAbstract only
Chapter 10 - Bacterial transformation using CaCl2
Pages 57-61 - Book chapterAbstract only
Chapter 11 - Transformation of E. coli by electroporation
Pages 63-67 - Book chapterAbstract only
Chapter 12 - Transfection of cells using lipofectamine
Pages 69-72 - Book chapterAbstract only
Chapter 13 - Total RNA isolation
Pages 73-78 - Book chapterAbstract only
Chapter 14 - RNA isolation from plant tissues with high phenolic compounds and polysaccharides
Pages 79-82 - Book chapterAbstract only
Chapter 15 - Total protein extraction with TCA-acetone and 2D gel electrophoresis
Pages 83-87 - Book chapterAbstract only
Chapter 16 - Dansyl-Edman method of peptide sequencing
Pages 89-92 - Book chapterAbstract only
Chapter 17 - ISSR analysis
Pages 93-96 - Book chapterAbstract only
Chapter 18 - Gene cloning/recombinant DNA technology
Pages 97-107 - Book chapterAbstract only
Chapter 19 - Polymerase chain reaction (PCR)
Pages 109-116 - Book chapterAbstract only
Chapter 20 - Blotting/hybridization and its types
Pages 117-122 - Book chapterAbstract only
Chapter 21 - Dot blot analysis
Pages 123-125 - Book chapterAbstract only
Chapter 22 - ELISA (enzyme-linked immunosorbent assay)
Pages 127-131 - Book chapterAbstract only
Chapter 23 - Study of principle of centrifugation
Pages 133-137 - Book chapterAbstract only
Chapter 24 - Study of principle of chromatography
Pages 139-141 - Book chapterAbstract only
Chapter 25 - Thin layer chromatography (TLC)
Pages 143-146 - Book chapterAbstract only
Chapter 26 - Gel-filtration or size-exclusion chromatography
Pages 147-149 - Book chapterAbstract only
Chapter 27 - Ion-exchange chromatography
Pages 151-154 - Book chapterAbstract only
Chapter 28 - To perform affinity chromatography
Pages 155-158 - Book chapterAbstract only
Chapter 29 - To perform paper chromatography
Pages 159-161 - Book chapterAbstract only
Chapter 30 - Growth of bacterial cultures and preparation of growth curve
Pages 163-166 - Book chapterAbstract only
Chapter 31 - To study standard operating procedures (SOPs) for various equipment
Pages 167-179 - Book chapterNo access
Index
Pages 181-186
About the book
Description
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work.
This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work.
This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.
Key Features
- Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology
- Features clear, step-by-step instruction for applying the techniques covered
- Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
- Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology
- Features clear, step-by-step instruction for applying the techniques covered
- Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Details
ISBN
978-0-12-824449-4
Language
English
Published
2021
Copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Imprint
Academic Press