Cover for Advanced Methods in Molecular Biology and Biotechnology

Advanced Methods in Molecular Biology and Biotechnology

A Practical Lab Manual

Book2021

Authors:

Khalid Z. Masoodi, Sameena Maqbool Lone and Rovidha Saba Rasool

Advanced Methods in Molecular Biology and Biotechnology

A Practical Lab Manual

Book2021

 

Cover for Advanced Methods in Molecular Biology and Biotechnology

Authors:

Khalid Z. Masoodi, Sameena Maqbool Lone and Rovidha Saba Rasool

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Book description

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotech ... read full description

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  2. Book chapterAbstract only

    Chapter 1 - Introduction to molecular biology techniques

    Pages 1-6

  3. Book chapterAbstract only

    Chapter 2 - Agarose gel electrophoresis

    Pages 7-12

  4. Book chapterAbstract only

    Chapter 3 - SDS-polyacrylamide gel electrophoresis

    Pages 13-20

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    Chapter 4 - Immunoelectrophoresis

    Pages 21-25

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    Chapter 5 - Plasmid DNA isolation using alkaline lysis method from E. coli DH5α cells

    Pages 27-32

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    Chapter 6 - Restriction digestion of plasmid DNA

    Pages 33-36

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    Chapter 7 - Genomic DNA extraction from the plant leaves using the CTAB method

    Pages 37-44

  9. Book chapterAbstract only

    Chapter 8 - Genomic DNA extraction from silkworm using modified phenol:chloroform method (Suzuki et al., 1972)

    Pages 45-50

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    Chapter 9 - Genomic DNA extraction from Fungal Mycelium using the modified Cenis, 1992 method

    Pages 51-56

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    Chapter 10 - Bacterial transformation using CaCl2

    Pages 57-61

  12. Book chapterAbstract only

    Chapter 11 - Transformation of E. coli by electroporation

    Pages 63-67

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    Chapter 12 - Transfection of cells using lipofectamine

    Pages 69-72

  14. Book chapterAbstract only

    Chapter 13 - Total RNA isolation

    Pages 73-78

  15. Book chapterAbstract only

    Chapter 14 - RNA isolation from plant tissues with high phenolic compounds and polysaccharides

    Pages 79-82

  16. Book chapterAbstract only

    Chapter 15 - Total protein extraction with TCA-acetone and 2D gel electrophoresis

    Pages 83-87

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    Chapter 16 - Dansyl-Edman method of peptide sequencing

    Pages 89-92

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    Chapter 17 - ISSR analysis

    Pages 93-96

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    Chapter 18 - Gene cloning/recombinant DNA technology

    Pages 97-107

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    Chapter 19 - Polymerase chain reaction (PCR)

    Pages 109-116

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    Chapter 20 - Blotting/hybridization and its types

    Pages 117-122

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    Chapter 21 - Dot blot analysis

    Pages 123-125

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    Chapter 22 - ELISA (enzyme-linked immunosorbent assay)

    Pages 127-131

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    Chapter 23 - Study of principle of centrifugation

    Pages 133-137

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    Chapter 24 - Study of principle of chromatography

    Pages 139-141

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    Chapter 25 - Thin layer chromatography (TLC)

    Pages 143-146

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    Chapter 26 - Gel-filtration or size-exclusion chromatography

    Pages 147-149

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    Chapter 27 - Ion-exchange chromatography

    Pages 151-154

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    Chapter 28 - To perform affinity chromatography

    Pages 155-158

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    Chapter 29 - To perform paper chromatography

    Pages 159-161

  31. Book chapterAbstract only

    Chapter 30 - Growth of bacterial cultures and preparation of growth curve

    Pages 163-166

  32. Book chapterAbstract only

    Chapter 31 - To study standard operating procedures (SOPs) for various equipment

    Pages 167-179

  33. Book chapterNo access

    Index

    Pages 181-186

About the book

Description

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work.

This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work.

This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.

Key Features

  • Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology
  • Features clear, step-by-step instruction for applying the techniques covered
  • Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
  • Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology
  • Features clear, step-by-step instruction for applying the techniques covered
  • Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Details

ISBN

978-0-12-824449-4

Language

English

Published

2021

Copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Imprint

Academic Press

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Authors

Khalid Z. Masoodi

Transcriptomics Laboratory, Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

Sameena Maqbool Lone

Division of Vegetable Science, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, India

Rovidha Saba Rasool

Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, India