Involvement of increased endoplasmic reticulum stress in the development of cataracts in BALB.NCT-Cpox^nct mice

Highlights

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Coproporphyrin accumulation promotes endoplasmic reticulum stress in the mouse lens.

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Coproporphyrin induces aggregation of crystallins and keratins in the lens.

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Coproporphyrin accumulation reduces crystallin production in the lens.

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Lens homeostasis is stuck in a vicious cycle that eventually leads to cataracts.

Abstract

The BALB.NCT-Cpox^nct is a mutant mouse model for hereditary cataracts. We previously uncovered that the primary cause of the cataracts of BALB.NCT-Cpox^nct is a mutation in the coproporphyrinogen oxidase (Cpox) gene. Because of the mutation, excessive coproporphyrin is accumulated in the BALB.NCT-Cpox^nct lens. In this study, we analyzed the changes in transcriptome and proteins in the lenses of 4- and 12-week-old BALB.NCT-Cpox^nct to further elucidate the molecular etiology of cataracts in this mouse strain. Transcriptome analysis revealed that endoplasmic reticulum (ER) stress was increased in the BALB.NCT-Cpox^nct lens that induced persistent activation of the PERK signaling pathway of the ER stress response. Also, levels of crystallin transcripts and proteins were reduced in the BALB.NCT-Cpox^nct lens. Analysis of proteins disclosed aggregation of crystallins and keratins prior to the manifestation of cataracts in 4-week-old BALB.NCT-Cpox^nct mice. At 12 weeks of age, insoluble crystallins were accumulated in the cataractous BALB.NCT-Cpox^nct lens. Overall, our data suggest the following sequence of events in the BALB.NCT-Cpox^nct lens: accumulated coproporphyrin induces the aggregation of proteins including crystallins. Aggregated proteins increase ER stress that, in turn, leads to the repression of global translation of proteins including crystallins. The decline in the molecular chaperone crystallin aggravates aggregation and insolubilization of proteins. This vicious cycle would eventually lead to cataracts in BALB.NCT-Cpox^nct.